Title: Effect of sub-hypothermia combined with nimodipine infusion on the global cerebral ischemic-reperfusion injury in rabbit
Abstract: Objective To investigate the effect of sub-hypothermial combined with nimodipine infusion on the expression of interllukine(IL)-1,tumor necrosis factor α(TNF-α)and IL-10 in plasma after the global cerebral ischemic/reperfusion(I/R)injury.Methods Forty healthy New Zealand rabbits were randomly divided into five groups.The model was made by occluding the bilateral common carotid arteries for 30 min combined with hypotension.Except normal group(group V),groups of Ⅰ,Ⅱ,Ⅲ,Ⅳ were ischemic for 30 min and reperfused for 3 hours,local cerebral sub-hypothermia was given to group Ⅱ for 3 hours combined with the same I/R,10 μg·kg-1·h-1 nimodipine was infused in group Ⅲ during ischemic treatment,and the sub-hypothermia combined with nimodipine infusion was given in group Ⅳ.The temperature,MAP and CVP were recorded and the arterial blood was taken before ischemia,at 30 min after ischemia,reperfusion for 30 min,1,2 and 3 hours.ELISA method was used to detect the IL-1,TNF-α,IL-10 expression in plasma.Results The water contant of the brain was significantly high in group Ⅰ,but the difference was not significant among the other four groups.The expression of IL-1 was increased at T1 and reached peak at T4,but the TNF-α was increased and reached peak at T1 in group Ⅰ,compared with T0,the difference was very significant(P0.01).The expressions of IL-1 and TNF-α were increased in T1 in group Ⅱ to Ⅳ(P0.01).The level was significantly higher in group Ⅰ than that in groups of Ⅱ,Ⅲ,Ⅳ,Ⅴ at T2 to T5(P0.01).In group Ⅱ,the levels of the IL-1 and TNF-α were little more than group Ⅳ,Ⅴ at T2 to T4,but lower than those in group Ⅰ(P0.01).The expression of IL-10 was decreased at T1 and the lowest at T5 in group Ⅰ,after the sub-hypothermia and/or nimodipine infusion in groups of Ⅱ,Ⅲ,Ⅳ,the expression of IL-10 was increased at T2 and maintained until at T5,compared with group Ⅰ,the difference was significant(P0.01).Conclusion The sub-hypothermia and sub-hypothermia combined with nimodipine infusion can reduce the expressions of IL-1 and TNF-α and increase the expression of IL-10 significantly,indicating that it may attenuate the inflammation response after global I/R injury in rabbits.
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot