Title: Research on Best Induced Conditions for Expression of Recombinant Fusion Protein
Abstract: [Objective] To study the best induced conditions for expression of recombinant fusion protein.[Method] The best initial OD600,induction amount,induction duration,induction temperature,ampicillin concentration,liquid volume and other culture conditions were explored by using parallel fermentation experiments with different technical indicators to improve the expression level of the recombinant fusion protein of IMPACT-CN pTYB11 vector.[Result] Expression of SDS-PAGE and Western-Blotting analysis showed that the recombinant fusion protein has a high immunogenicity.[Conclusion] This study laid the foundation for further protein purification in diagnosis,production of diagnostic kits with protein and clinical application.
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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