Title: Research of induction of donor-specific tolerance with sublevel dose irradiation
Abstract: [Objective] To study the effect on the immunologic reaction of limb allotransplantation by using method of intra-bone marrow injection of donor bone marrow cells combined with sublevel dose irradiation and Fludarabine. [Method] The inbred SD rats were chosen as donors and inbred Wistar rats as recipients. 40 recipients were divided into four groups randomly after limb alltransplantation. Rats in group A received transplantation only; group B rats received irradiation in the sublethal level and Fludarabine the day before transplantation. In group C, bone marrow cells were directly injected into the intra-bone marrow cave of recipients immediately after transplantation. In group D, using a combination of the injection of Fludarabine,irradiation and injection of donor bone marrow cell. The condition of survival and rejection of grafts was observed. Tolerance rats in group D were received SD and DA rat skin grafting and analysis of cytokine mRNA expression. [Results] Compared with other groups, mean survival time of limb allografts was prolonged obviously in group D (P 0.01)(120 d). Rats in group D had a low reaction to the transplanted SD skins, but had strong reactions to DA rat′s skin. The levels of IL-2 and IFN-γ mRNA expression in tolerant rats in group D were much lower than those in the control rats. The levels of IL- 4 and IL-10 mRNA in tolerant rats in group D were much higher than those in the control rats. [Conclusion] Combination method can induce donor-specific tolerance in limb alltransplantation in rats.
Publication Year: 2008
Publication Date: 2008-01-01
Language: en
Type: article
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