Title: Cloning and expression of porcine IL-4 gene
Abstract: Using RT-PCR,pig IL-4 gene was cloned from porcine PBMC stimulated by LPS and PHA,After sequencing,compared with IL-4 gene published in GenBank,the homologies of the nucleotide acide sequence and amino acide sequence were 99?% and(97.8?%),respectively.Then the recombinant expression plasmid(pGEX-IL-4) was constructed,pGEX-Il-4 was expressed in E.coli by IPTG induction,SDS-PAGE analysis showed that the expression product was fusion protein with molecular weight of 38 ku and the percentage of expression protein in E.coil protein was 30?%.This has laid a foundation for the production and vaccine adjuvant development of porcine IL-4.
Publication Year: 2005
Publication Date: 2005-01-01
Language: en
Type: article
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