Title: Antagonizing effect of ISA on lipid peroxidation of vein endothelial cells
Abstract: Objective To study the influence of 3, 4-oxo-isopropylidene-shikimic acid (ISA) on lipid peroxidation of human umbilical vein endothelial cells (HUVEC) induced by hydrogen peroxide (H2O2) in vitro. Methods The concentration of malonaldehyde (MDA) in HUVEC was detected by thiobarbituric acid (TBA) method, the activities of superoxide dismutase (SOD) was detected by xanthine oxidase system and catalase (CAT) was detected by visible spectrum assay. The clearance (C) of superoxide anion and OH radicals by ISA were tested by free radical prehensile experiment in vitro.Results The lipid peroxidation induced by H2O2 (200 μmol/L for 6 hours) was alleviated obviously in HUVEC cultured with ISA (10-5, 10-4 mol/L) for 6 hours, MDA was decreased and the activities of CAT and SOD were protected. The activities of SOD and CAT were improved after HUVEC incubation with ISA for 12 hours. ISA (10-5~10-3 mol/L) had an obvious clearing effect on superoxide anion, showed a relationship between quality and effectiveness. ISA (10-3 mol/L) could also clear OH radicals.Conclusion ISA has an antagonizing effect on lipid peroxidation of vein endothelial cells in vitro, but no direct influence on the activities of SOD and CAT.
Publication Year: 2007
Publication Date: 2007-01-01
Language: en
Type: article
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Cited By Count: 3
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