Title: Response Surface Methodology for Optimization of Extraction Conditions of Total Phenolic Compounds from Garlic and Determination of Antioxidant Activity
Abstract: Response Surface Methodology(RSM) was used to optimize extraction parameters(including material/liquid ratio,extraction time and concentration of extracting agents) so as to achieve the maximum extraction yield of total phenolic compounds.Studies were carried out on the respective independent variables(material/liquid ratio,extraction time and concentration of extracting agents) and the interactive effects of these three factors on the total phenolic compounds according to Box-Benhnken design.A mathematical quadratic polynomial regression equation reflecting the relationship between the total phenolic compounds and the above extraction parameters was established.The optimal extraction parameters were obtained as: material/ liquid ratio 1:14(g/mL),extraction time 31 min,and 80% acidification methanol as extracting agent.Under the optimal condition,the total phenolic content was 0.67 mg GAE/g,and the model prediction deviation was 6.9%.Meanwhile,the antioxidant activity was determined with five different methods,and the results showed that garlic had good antioxidant activity.
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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