Title: Mutation of p53 gene in esophageal squamous cell carcinoma
Abstract:Objective To characterize expression and mutation of p53 in esophageal squamous cell carcinoma(ESCC), and to test the reliability of immunohistochemistry for p53 over-expression as an indicator of p53...Objective To characterize expression and mutation of p53 in esophageal squamous cell carcinoma(ESCC), and to test the reliability of immunohistochemistry for p53 over-expression as an indicator of p53 mutation in this context. Methods Fifty-six ESCC patients were selected from a high risk population in Northern China. ESCC were sampled from 95% ethanol-fixed, paraffin-embedded tissue by laser microdissection. Mutations in p53 exons 4 to 9 were detected by polymerase chain reaction-strand conformation polymorphism (PCR-SSCP) and confirmed by direct DNA sequence. Nuclear accumulation of p53 protein was analyzed immunohistochemically from tissue sections. Results p53 gene mutations were found in 43 (77%,43/56). The majority of mutations observed were in exon 5, where the most common type of nucleotide subsititution was a G:C→A:T or C:G→T:A transition, one half of which occurred at CPG sites. p53 protein accumulation was found in 26 cases (46%,26/56), including 23 (23/26) cases were detected p53 mutation. A high concordance rate of 95% (53/56) was found between p53 gene mutation and p53 protein accumulation. Conclusion p53 gene mutations may play an important role in the carcinogenesis of human esophageal cancer. Mutational analyses of p53 by PCR-SSCP and p53 accumulation by immunohistochemistry are mostly concordant with ESCC.Read More
Publication Year: 2006
Publication Date: 2006-01-01
Language: en
Type: article
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