Title: Effect of formaldehyde on the proliferation activity and apoptosis of leukemia cells
Abstract: Objective To investigate the effect of formaldehyde on the proliferation activity and apoptosis of leukemia cells.Methods HL60 and K562 cells were incubated with formaldehyde at different concentrations.The proliferation activity was analyzed by MTT assay and cell apoptosis was detected by fluorescence microscopy and flow cytometry.Results With 10 or 15 μmol/L formaldehyde,HL60 and K562 cells proliferated slightly.20 μmol/L formaldehyde could inhibit cell proliferation activity with concentration and time dependence.The proliferation activities of 86.13% HL60 cells and 91.16% K562 cells were inhibited when the cells were incubated with 100 mmol/L formaldehyde for 48 hours.Almost no apoptosis expression was observed in cells of the control and 10 μmol/L formaldehyde group when cultured for 24 hours,while they could be observed in cells of 30 and 100 μmol/L formaldehyde groups,especially in the latter.Compared to the control,the apoptosis rate in 10 μmol/L formaldehyde groups was not significantly changed(P0.05).The early apoptosis rate in 30 μmol/L formaldehyde group had no significant differences with the control and 10 μmol/L formaldehyde group,but the late apoptosis rates had obviously increased(P0.05).The apoptosis rate markedly enhanced in cells of 100 μmol/L formaldehyde group(P0.05).Conclusion Low concentration of formaldehyde can elicit proliferation of acute leukemia cells without apoptosis induction,while high concentration of formaldehyde can inhibit the proliferation of leukemia cells and induce apoptosis.
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
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