Title: Effects of Melatonin on HO-1 Expression in Mice with Acute Renal Ischemia-reperfusion Injury
Abstract: OBJECTIVE:To study protection effect of melatonin(MT) on mice with acute renal ischemia-reperfusion injury(IRI) and its mechanism.METHODS:A total of 40 Kunming mice were divided into MT high-dose and low-dose groups(10,1 mg·kg-1),sham operation group,IRI group.Four groups were all injected with relevant drug intraperitoneally 30 min before modeling.Last two groups received 3% ethanol normal saline.The level of serum cretinine(Cr) and Bun were determined,and renal pathologic changes were observed 24 hours after modeling.The heme oxyenase-1(HO-1) expression of kidney was evaluated by immunohistochemistry.RESULTS:Compared with sham operation group,some ischemic changes of renal tubular epithelical cells was observed,and the content of Cr and Bun(P0.01) and the HO-1 expression increased significantly in IRI group.Compared with IRI group,some mild ischemic changes of renal tubular epithelical cells was observed,the contents of Cr and Bun were decreased(P0.01) while the HO-1 expression was increased significantly in MT high-dose and low-dose groups.CONCLUSION:MT could increase HO-1 expression to protect mice with IRI.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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