Title: Abnormal Expression and Clinical Significance of Interleukin 10 in Patients with Systemic Lupus Erythematosus
Abstract: Objective:To evaluate the significance of abnormal expression of interleukin 10(IL 10) in systemic lupus erythematosus and the relationship between IL 10 aberrant expression and lupus predisposition, disease activity and prognosis. Methods:ABC ELISA was used to analyze IL 10 serum levels, and LightCycler fluorescent quantification system was used to validate the aberrant expression of IL 10 mRNA.Results:Either the serum IL 10 levels or IL 10 mRNA expression was significantly increased in patients with SLE compared with the control.There was a positive linear correlation between serum IL 10 and IL 10 mRNA expression in PBMCs. IL 10 levels measured in serum and in supernate of cultured PBMCs in SLE patients with positive anti dsDNA antibody or with lupus nephritis were significantly higher than those measured in patients with negative anti dsDNA antibody or without lupus nephritis respectively. Although IL 10 levels of active patients were higher than those of inactive ones, there was no significant difference in statistics; furthermore the IL 10 levels are not correlative to the SLEDAI. The IL 10 level in cultured PBMCs supernate of SLE patients, whether it was spontaneously secreted or after stimulation by PHA, was significantly higher than that in normal control. Conclusion:Abnormally high expression of IL 10 may contribute to the pathogenesis of SLE, especially to certain damages,such as lupus nephritis.
Publication Year: 2002
Publication Date: 2002-01-01
Language: en
Type: article
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