Title: Determination of Quercitrin in Hypericum japonicum Thunb.by HPLC
Abstract: OBJECTIVE To determine the content of quercitrin in Hypericum japonicum Thunb.by HPLC.METHODS An Agilent C18 column(250 mm×4.6 mm,5μm)was used.The mobile was acetonitrile-0.05 mol·L -1potassium dihydrogen phosphate(19∶81),and detected at 256 nm with flow rate of 1.0 mL·min -1 .RESULTS A good linearity was observed for quercitrin in the range of 0.120 4-2.408μg.The average recovery of quercitrin was 96.0%with RSD of 1.3%(n=6). CONCLUSION The method is convenient,stable,reliable and special for the dermination of quercitrin in Hypericum japonicum Thunb..
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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Cited By Count: 1
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