Title: Neuroprotective effect of Batroxobin on rats with perinatal hypoxic-ischemic brain damage
Abstract: ObjectiveTo study neuroprotective effect of batroxobin on r ats with perinatal hypoxic-ischemic brain damage (HIBD). MethodsA animal model was established by blocking bilateral uterine artery 20 minutes of pregnant rats at full-term. The young rats suffered with HIBD were randomly divided into two groups at the same time. One is hypoxic -ischemic (HI) group,the other is batroxobin intervention group (batroxobin 8BU/kg,ip,inject ed at 30min,24h and 48h after birth,together three times). The normal control group was set up meanwhile. Hypoxic-ischemic group and normal control group were injected with equil dose o f salt solution of 9 percent at the same time. The young rats of three groups were killed at 72h ,7d,14d,21d. The brain water content and the weight of brain were measured. T he number of nerve cell apoptosis was observed by terminal deoxynucleotidyl transferase mediated deoxyur idine triphosphate biotin nick end labeling (TUNEL) in situ. ResultsThe brain water content and the weight of brain in HI group were higher than that in batroxobin intervention group and normal control group at 72h ( P 0.05). The brain water content was no difference at other time among three group. The weight of brain in HI group were lower than that in batroxobin intervention group and normal control group at 14d and 21d ( P 0.05). The weight of brain in batrox obin intervention group were lower than that in normal control group at 14d and 21d ( P 0.05). The number of apoptosis in HI group was higher than that in batroxobin intervention group and normal contro l group during 72h~14d ( P 0.05). The number of apoptosis was no difference at 21d among three group. The number of apoptosis in batroxobin intervention group was higher than that in normal con trol group during 72h~7d ( P 0.05). ConclusionBatroxobin can obviously alleviate brain edema of convalescent period and brain atrophy of impatient period in rats suffered w ith HIBD. Batroxobin can be neuroprotective function by anti-apoptosis.
Publication Year: 2004
Publication Date: 2004-01-01
Language: en
Type: article
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