Title: Inhibitory effect of IL-10 on induced expression of ICAM-l in renal tubular cells is mediated by down regulated activity of nuclear factor kB
Abstract: Objecive To explore the effect of interleukin-10(IL10) on tumor necrosis factor-α(TNFα)- induced expression of intercellular adhesion molecule 1 (ICAM-1 )and related transcription pathway in human renal tubular cells. Methods Human renal proximal tubular cell line HK-2 cell was used as target cell. ICAM-1 was measured by cell ELISA and Northern blot in protein and mBNA levels. Activities of transcriptional factors nuclear factor GB(NFkB) and activator protein 1 (AP-1) were determined by electrophoresis mobility shift assay. Results TNFα-induced ICAM-l expression in HK-2 cells was increased in both protein and mNRA levels(P 0.01 ). NFkB inhibitor N-tosyl phenyalanine chlormethyl ketone (TPCK)could inhibit these effects of TNLFα. TNFα had no effect on AP-l activity with doses of 1 ng/ml to 100 ng/ml and incubation periods of one to four hours. IL-10 iuhibited TNFα-induced ICALM-1 protein expression in dosage from 1 ng/ml to 20 ng/ml(decreased by 25 .7% to 35. 1 %, P 0.05), as well as ICAM-1 mRNA expression and activity of NFkB in HK-2 cells. Conclusions TNFα induces ICAM-l expression in HK-2 cells through activation of NFkB. Inhibitory effect of IL-10 on ICAM-l expression induced by TNFα is mediated by, at least in a part, down-regulation of NFkB activity.
Publication Year: 2000
Publication Date: 2000-01-01
Language: en
Type: article
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