Title: Study on replication deficient adenoviral vectors mediated enhanced green fluorescent protein transduction to neural stem cells
Abstract: Objective:To study the feasibility and infection efficiency of replication deficient adenoviral vectors(AdVec) mediated enhanced green fluorescent protein(EGFP) transduction to neural stem cells(NSCs).Methods:Rat′s NSCs were cultured in vitro,AdVec-EGFP virus were amplified and purified.NSCs were transduced with AdVec-EGFP virus and results were estimated with fluorescence microscope and immnocytochemistry.Results:Report gene EGFP could be high performed in infected NSCs;fluorescein started to be expressed 6 hours after trasfected,and reached peak time afer 48 hours.The infection rate was(76±2)%;the biological characters of NSCs had no changes.Conclusions:AdVec-EGFP has higher gene expression efficiency of mediating exogenous gene;it is a better labelled compound for NSCs investigation both in vivo and in vitro.
Publication Year: 2007
Publication Date: 2007-01-01
Language: en
Type: article
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