Title: Effects of lactuside B on the expression of VEGF mRNA and protein in the rats' hippocampus after ischemic stroke
Abstract: OBJECTIVE To explore the effect of extractive lactuside B( LacB) from Pterocypsela elata on the expression of hippocampus VEGF mRNA and protein in the ischemic stroke rats. METHODS SD male rats were randomly divided into six groups: Sham operation group,model group,positive group,LacB low dose( 12. 5 mg·kg-1) group,LacB middle dose( 25 mg·kg-1) group and LacB high dose( 50 mg·kg-1) group. All the rats received intra-peritoneal injections twice a day with a dosage of 5 mL·kg-1. Seven animals from each group were sacrificed at 24 h,four rats were used for detection of VEGF protein expressions in hippocampus by immunohistochemistry method,and three rats were used for detection of VEGF mRNA expressions by RT-PCR technique. The rest animals were sacrificed at 72 h after giving medications,four rats were used for immunohistochemistry technology,and others were used for RT-PCR. RESULTS After reperfusion for 24 h,model group VEGF gene and protein expression increased significantly compared with the sham group in the hippocampus( P 0. 01),but after reperfusion for 72 h VEGF mRNA and protein expression decreased. VEGF mRNA and protein expression of all the LacB groups increased after giving drugs,which was significantly different from that of model group( P 0. 05,P 0. 01). 72 h after drug administration,VEGF expression of medium and high dose group had more VEGF expression,while those of the model group and positive drug group were significantly different( P 0. 05,P 0. 01). CONCLUSION LacB could ameliorate ischemic stroke through promoting early neurogenesis and formation of the late microcirculation in ischemic penumbra and hippocampal sensitive area,which may be attributed to its VEGF expression induction ability in the hippocampus.
Publication Year: 2014
Publication Date: 2014-01-01
Language: en
Type: article
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