Title: Effects of propofol on the kidney injury induced by intestinal ischemia/reperfusion in rats
Abstract: Objective:To investigate the effects of propofol on the kidney injury induced by intestinal ischemia/reperfusion (I/R) in rats and the underlying mechanism. Methods: Twenty-four Wistar rats of male weighing 280~330 g were randomly divided into 3 equal groups (n=8 each). Superior mesenteric artery (SMA) were separated from abdominal cavity and continuously injected 0.9% Sodium Chloride 10 ml/(kg·h) by pump in sham operation group (S). Intestinal ischemia/reperfusion was produced by clamping SMA for 1 h followed by 2 h reperfusion and continuously injected 0.9% Sodium Chloride 10 ml/(kg·h) by pump in I/R group . Propofol group (P) received propofol 10 mg/(kg·h) intravenously 5 min before reperfusion. We used instrument to determine BUN and Bcr from venous blood. Both kidneys were taken out of rats which were sacrificed by decapitation at the end. Cortical part of the right kidney was made into homogenate, measuring the determination of SOD activity and MDA content. The left was conserved by 10% Liquor Formaldehyde. The structural changes of kidney were examined by microscopy with HE staining. Results: The SOD activity was significantly lower while the MDA content was higher in I/R group than in S group (P0.05). The SOD activity was significantly higher while the MDA content was lower in P group than in I/R group (P0.05). Under the microscope, we found that glomcrulus and postglomerular capillary were expanded, cellularedema, ruptured erythrocyte were accumulated in postglomerular capillary. Conclusion: Intestinal ischemia/reperfusion can induce kidney injury, but propofol can suppress this injury.
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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