Title: Determination of gallic acid in megranates pericarp with HPLC
Abstract: OBJECTIVE To determine the content of gallic acid in megranate's-pericarp with HPLC.METHODS A zorbax C-18 column(250mm×4.6mm,5μm) was used.The mobile phase was water-dimethyformamide-glacial acetic acid(99.82∶0.15∶(0.03)),and detected at 270nm;with flow rate of 1.0mL·min~(-1),and the temperature of column was at room temperature. RESULTS The linearity of gallic acid was over the range of 0.081-0.811μg·mL~(-1) with the correlation coefficient 0.999 9.The average recoveries of different concentration was 98.35% with RSD 1.63%(n=6);the within-day and between-day RSD were 1.01% and 1.60%(n=6),respectivelity.CONCLUSION The method is convenient,stable,reliable and special for the dermination of gallic acid in Megranate's-pericarp.
Publication Year: 2006
Publication Date: 2006-01-01
Language: en
Type: article
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