Title: Optimum condition of isolation and preparation of polyphenol in Camellia pericarp by semi-preparation HPLC
Abstract: In order to improve preparation quantity and separation degree,the preparation condition of polyphenol in Camellia pericarp was optimized by semi-preparation HPLC.The results showed that after extracting polyphenol with 50% of alcohol,the optimization condition of isolating and purifying polyphenol by semi-preparation HPLC was as follows: gradient elution was carried on by mobile phase A(volume ratio of water to acetic acid was 100︰1) and B(acetonitrile) at 2.5 mL/min flow rate,the injection volume was 0.4 mL.The content of single polyphenol was 21.22 mg/g through the preparation method,and it was effective and reliable.
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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