Title: Effects of Double-Reinforcing and One-Unblocking of Acupuncture Method on Hippocampus Synaptic Structure and Content of BDNF and Expression of PKC mRNA in Aging Rats with Yang Deficiency
Abstract: Objective To observe the effects of Double-reinforcing and One-unblocking of acupuncture on hippocampus synaptic structure,the content of BDNF,and the expression of PKCmRNA of aging model rats with Yang-deficiency. Methods 48 SD rats were randomly divided into four groups: Normal Group,Model Group,Double-reinforcing and One-unblocking of electro-acupuncture Group( EA),and electro-acupuncture control Group( EC). Every rats in Model Group,EA Group,and EC Group were injected with D-galactose subcutaneously for 40 days,to induce the sub-acute aging model rats. Then except the rats in Normal Group,all rats were injected with hydrocortisone for 7days,to induce the Yang-deficiency symptom on the aging model rats. The rats in Normal Group were injected of same amount of saline. Rats in EA Group were treated with Double-reinforcing and One-unblocking of electro-acupuncture,and EC Group were stimulated with YintangZhongjiYinLingquan,each treatment were last for 4 weeks. Synaptic structural parameters were analysised by electron microscopy and biological image analysis system; detect the content of BDNF by radioimmunity method; RT-PCR examines the PKC mRNA expression.Results In comparison with the Normal Group,the length of active zones and the thickness of PSD are all decreased,the synaptic cleft width enlarged significantly in the Model group( P 0. 01),the content of BDNF declined remarkably in Model Group( P 0. 05),the expression of PKCmRNA declined remarkably in Model Group( P 0. 01); In comparison with the Model Group,the thickness of PSD increased,and the synaptic cleft width decreased remarkably in the EA Group( P 0. 01),the content of BDNF in EA Group increased( P 0. 05),the expression of PKCmRNA raised remarkably in EA Group( P 0. 01); In comparison with the EA Group,the length of active zones of PSD decreased in EC Group( P 0. 05),the content of BDNF in EC Group decreased( P 0. 05),the content of PKCmRNA in EC Group decreased( P 0. 05). Conclusion The above results suggest that the effects of Double-reinforcing and One-unblocking of acupuncture method can improve the hippocampal synaptic structural parameters; and the treatment can adjust and regulate content of BDNF and the PKC mRNA expression in aging model rats with Yang-deficiency.
Publication Year: 2013
Publication Date: 2013-01-01
Language: en
Type: article
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