Title: Determination of Berberine Chloride in Mouse Plasma after Intragastric Administration of Lianxiang Microemulsion
Abstract: OBJECTIVE To determine berberine chloride in mouse plasma for the investigation of the bioavailability of Lianxiang microemulsion in mouse. METHODS After single dose ig administration,the plasma samples were taken at different time,respectively, for the determination of berberine chloride by HPLC; And the relative bioavailability of Lianxiang microemulsion vs Lianxiang emulsion (reference formulation) was calculated. RESULTS Berberine chloride was separated well from endogenous foreign substances. The calibration curve of berberine chloride was liner over the range of 10.4-156 microg/L (r = 0.9991) and the method recovery was within 89.6%-94.7%. The intraday RSD or the inter-day RSD 24h of the microemulsion and the emulsion were (688.3 +/- 123.7) and (371.4 +/- 68.4) microg x h/L,respectively; And the relative bioavailability of the microemulsion vs the emulsion was (193.1 +/- 63.2)%. CONCLUSION The validated HPLC method is suitable for the determination of berberine chloride in mouse plasma; And microemulsification can clearly promote the bioavailability in mouse of berberine chloride in Lianxiang prescription.
Publication Year: 2010
Publication Date: 2010-06-01
Language: en
Type: article
Indexed In: ['pubmed']
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Cited By Count: 3
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