Title: Effects and mechanisms of high concentration oxygen inhalation on lung of newborn rats
Abstract:Objective: To observe the pathologic and biochemical changes of lung by mimicking the status of premature newborn in high concentrated oxygen and to discuss the effects and mechanisms of oxygen inhala...Objective: To observe the pathologic and biochemical changes of lung by mimicking the status of premature newborn in high concentrated oxygen and to discuss the effects and mechanisms of oxygen inhalation on the lung. Methods: Newborn rats were put into the oxygen box with the oxygen concentration of 60% continuously for 1,4, and 7 days separately. Rats born at the same time which were fed in air condition served as control. Arterial blood gas analysis including, superoxide dismutase(SOD), malondialdehyde(MDA), nitric oxide(NO), and NO synthase (NOS) of kidney were meatured. The lung was observed by using HE staining. Results: The arterial partial pressure of oxygen in the 3 experimental groups were all significantly higher than that in control (P0.05), SOD was significantly lower than that in control (P0.05 ),and MDA was significantly higher than that in control (P0.05, P0.01, P0.01). Both NO and NOS were significantly higher in experimatal group than those in control (NO: P0.05,P0.01,P0.05; NOS: P0.05). The lung in 1 day old rats of experimental group showed no obvious pathologic change. While in the 4 day old rats, few red blood cell exudated in the alveoli and the septum thickened which were developed more obvious in the 7 day old rats with bleeding in the septum, inflammatory cell infiltrated and even alveoli fusion. No change was found in the control group. Conclusion: Superoxide free radical may play an important role in the mechanisms of pulmonary damage during high concentration oxygen supplying.Read More
Publication Year: 2004
Publication Date: 2004-01-01
Language: en
Type: article
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