Title: Dynamic expressions of MMP-9 and TIMP-1 protein and mRNA in the lung tissue of neonatal rats after lasting hyperoxia
Abstract:Objective: To explore the dynamic expressions of matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in the lung tissue of neonatal rats with hyperoxia-induced chro...Objective: To explore the dynamic expressions of matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in the lung tissue of neonatal rats with hyperoxia-induced chronic lung disease (CLD). Methods: Full-term newborn rats were continuously exposed to oxygen (0.90-0.95) or room air within 12 hours after birth. The changes in MMP-9 and TIMP-1 protein and mRNA expressions were measured by immunohistochemical method and reverse transcription polymerase chain reaction on days 1,3,7,14,and 21. Results: The expressions of MMP-9 protein and mRNA on day 3 were significantly higher in hyperoxia group than in room air group (P 0.05,P 0.01),but no between-group difference was found at other time points. The expression of TIMP-1 protein on days 3 and 7 was higher in hyperoxia group than in room air group,and on days 14 and 21 it was significantly higher in hyperoxia group than in room air group. The expression of TIMP-1 mRNA on days 3 and 7 was higher in hyperoxia group than in room air group,and on days 14 it was significantly higher in hyperoxia group than in room air group. No between-group difference in the expression of TIMP-1 mRNA was found on day 21. Conclusion: With the prolongation of hyperoxia,the expressions of MMP-9 and TIMP-1 change inconsistently. The balance between MMP-9 and TIMP-1 is disturbed,which may play an important role in the collagen deposition in lung interstitium and the inhibition of alveolar development in CLD.Read More
Publication Year: 2007
Publication Date: 2007-01-01
Language: en
Type: article
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