Title: Establishment of T lymphocyte clone of recipient and analysis on its donor-antigen-specificity
Abstract:Objective The aim of this study was to analyze the antigen-specificity of the clone after the recipient-against-donor antigen-specific T lymphocytes clones were formed.Methods The recipient rats were ...Objective The aim of this study was to analyze the antigen-specificity of the clone after the recipient-against-donor antigen-specific T lymphocytes clones were formed.Methods The recipient rats were immunized by the donor rats′spleencytes,then their T cells were isolated and purified.The T cells were cultured by adding raise cells,ConA or IL-2 at different concentrations and formed the T cell clone in the last.The changes of the ultrastructure,cell multiplication and subgroup of leukemic cells were investigated when the rats were immuned.Results A stable recipient-against-donor antigen-specific T cell clone was established;statistical data also verified that the difference between the immune groups and the comparison group was very significant (tt 0.05).Conclusion Mature T cell is able to proliferate to form clone in a suitable condition.Through stimulating the donor spleen cell antigen specificity the experiment proved that the finally-screened T-cell clone has an antigen-specificity to the donor.Read More
Publication Year: 2003
Publication Date: 2003-01-01
Language: en
Type: article
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