Title: Biological characteristics and antitumor activities of human cytokine-induced killer cells from patients with hepatocellular carcinoma
Abstract: 【Objective】 To compare the biological characteristics and anti-cancer activities of human cytokine-induced killer(CIK) cells from patients with hepatocellular carcinoma and healthy donors.【Method】 Peripheral blood mononuclear cells from patients with hepatocellular carcinoma and healthy blood donors were incubated in vitro and induced into CIK cells in the presence of various cytokines,such as interferon-gamma,interleukin-1,IL-2,and monoclonal antibody against CD3.The phenotype and proliferation activity of CIK cells were identified by flow cytometric analysis.The cytotoxicity of CIK cells in vitro was determined by MTT assay.The anti-tumor activities of CIK cells were evaluated after chemotherapy in BALB/c nude mice with SMMC-7721 liver cancer.【Result】 CIK cells from both groups didn't show obvious differences in phenotype,proliferation activity,cytotoxicity,and auxiliary therapeutic effects.CIK cells enhanced the effects of the chemotherapy in liver cancer model.【Conclusion】 The biological characteristics and antitumor activities of CIK cells from patients with hepatocellular carcinoma were almost the same as those from healthy donors.
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
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