Title: Study on mechanisms of doxorubicin-induced multidrug resistance in leukemia cell line K562
Abstract: Objective: To investigate doxorubicin-induced multidrug resistance(MDR) in human leukemia cell line K562 and then the mechanisms of drug resistance were studied.Methods: K562 cells were treated with doxorubicin at different concentrations and times.The expression of mdr-1 and other related genes were examined by reverse transcription-polymerase chain reaction(RT-PCR).P-glycoprotein(P-gp) was detected by flow cytometry(FCM) and the protein Survivin was analysed by immunofluorescence. Results: Incubation of K562 cells with doxorubicin not only induced functionally mdr-1 overexpression,but also increased the expression of other genes including MRP,Topo Ⅱ,YB-1,NF-kappaB and Survivin.Increasing level of mdr-1 mRNA correlated with its corresponding P-glycoprotein level in drug-induced cells.Survivin,a member of IAP family proteins,was also high expressed after incubation of K562 cells with doxorubicin. Conclusion: Doxorubicin can induce functional multidrug resistance overexpression in K562 cells in dose and time dependent manner,and the functional multidrug resistance may be due to the abnormal expression of genes MRP,Topo Ⅱ,YB-1,NF-kappaB and the anti-apoptosis gene Survivin.
Publication Year: 2007
Publication Date: 2007-01-01
Language: en
Type: article
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