Title: Protective effect of ventilation during warm ischemia on rabbit lung in non-heart-beating donors
Abstract: Objective To investigate the effect and mechanism of ventilation during warm ischemia on rabbit lung in non heart beating donors without heparinization. Methods Twelve Japanese big ear rabbits as donors were randomly divided into 2 groups. They were sacrificed with an intravenous injection of potassium chloride on the condition of non heparinization. In control group ( n =6), the ventilation was not given during warm ischemia. In the treatment group ( n =6), the ventilation was kept during warm ischemia. The rabbit's lung was flushed with cool Euro Collins solution when the warm ischemia lasted 1 h. Donor lungs were retrieved and stored at 4 ℃ for 2 h . Through the establishment of the isolated rabbit lung reperfusion model, the hemodynamics, lung cell viability, ventilatory, W/D and morphology were evaluated. Results The mean pulmonary artery pressure, pulmonary vascular resistance and W/D were significantly lower in the treatment group in the control group. On the other hand, the lung cell viability and P aO 2 were significantly greater in the treatment group than in the control group. Histologic examination showed that there were significantly less thrombsis in pulmonary vascular and relatively milder damage in lung parenchymal in the treatment group than in the control group. Conclusion The ventilation during warm ischemia has markedly protective effects on rabbit lung in non heart beating donor without heparinization.
Publication Year: 2004
Publication Date: 2004-01-01
Language: en
Type: article
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