Title: Study on vitrification of porcine GV stage oocytes
Abstract: In order to investigate an approach to improve the cryopreservation efficiency of porcine GV stage oocytes,three kinds of cryoprotectants(EDS,EFS40 and ES) were respectively used for the treatment of pre-and post-vitrification of oocytes.Then,with the ES chosen for vitrification solution,the routine straw method,OPS method and electron microscopy grid(EMG) method were adopted to cryopreserve porcine GV stage oocytes.Finally,ES was used as vitrification solution and OPS method as vitrification carrier,and porcine GV stage oocytes were divided into 5 groups for the treatment: the control,CB+centrifugation group,the group of vitrification only,CB + vitrification group,and CB + centrifugation + vitrification group.The toxic experiment of different cryoprotectants on porcine GV stage oocytes showed that ES solution showed the lowest toxicity and there was no significant difference compared with the control(P0.05).Among 3 types of freezing carriers,OPS method yielded significantly higher post-warming survival rate than EMG and straw method did(65.4% vs 45.0% and 38.6%,P0.05),and resulted in the optimal post-warming maturation rate(43.3%) as well.Porcine GV stage oocytes treated with cytochalasin B and centrifugation separately didn't show significant influence on decrease or increase in survival.However,their cleavage rate would be significantly lower than that of the control(39.2% vs 52.1%,P0.05).The pre-treatment with cytochalasin B or cytochalasin B + centrifugation could not improve the post-warming survival and developmental rates of vitrified GV stage oocytes.The OPS vitrification of porcine GV stage oocytes without other treatments could result in 7.8% of cleavage rate and subsequent morula development.
Publication Year: 2007
Publication Date: 2007-01-01
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot