Title: Osteoinduction of human bone morphogenetic protein 2 gene
Abstract: Objective:To prepare a biomaterials which can stimulate osteogenesis. Method:The BMP2-pcDNA3.1 plasmid was appended to a kind of spongy scaffold made of chitosan and collagen. The osteoblasts and fibroblasts of Wistar rats were seeded in the spongy scaffold separately. Then the cells-scaffold composites was cultured in vitro. At the same time, the scaffold materials were implanted subcutaneously into BALBc mice. The spongy scaffold appended pcDNA3.1 plasmid was treated as control scaffold material. After10 days, the cells-scaffold composites and the subcutaneous implants were taken out. The alkaline phosphatase activity and Ca content in these samples were detected separately. Result:Compared with the control group, the alkaline phosphatase activity and Ca content of the cells-scaffold composites both increased remarkably. The alkaline phosphatase activity and Ca content of the subcutaneous implants were increased by 61.5% and 16.2% separately.Conclusion:After appended BMP2 gene, the spongy scaffold material made of chitosan and collagen can stimulate osteogenesis.The study may provide a new method for osteoinduction of bone tissue engineering.
Publication Year: 2006
Publication Date: 2006-01-01
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot