Title: Study on the Expression and Bioactivity of Chick IL-2
Abstract: A pair of primers with restriction enzyme for prokaryotic expression were designed respectively according to the gene's CDs complete sequence.The gene was amplified by RT-PCR,then cloned into pGEM-T vector and transformed into the JM109 competent cells,the plasmid of white spot was identified by PCR and sequenced.The positive plasmid was digested by the restriction enzyme.The objective gene fragment was retrieved and cloned into expressing vector pGEX-6p-1 and transformed into BL21 competent cells.The plasmid of white spot was identified by PCR and restriction enzyme of endonucleases.The fusion proteins was expressed by different inducing conditions of IPTG,and purified by Sepharose 4B chromatography.The specific band was identified by Western blot analysis.The biological activity was tested by lymphocyte proliferation test.
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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