Title: Direct detection of Mycobacterium tuberculosis drug-resistant genes in clinical strains isolates by PCR-line probe assay
Abstract:Objective:To design the line probe assay for the detection of the rpoB,katG,rpsL,rrs,embB genes in drug-resistant Mycobacterium tuberculosis.Methods:The traditional cultivation,species identification ...Objective:To design the line probe assay for the detection of the rpoB,katG,rpsL,rrs,embB genes in drug-resistant Mycobacterium tuberculosis.Methods:The traditional cultivation,species identification and drug-susceptibility testing of 50 M.tuberculosis were performed in the clinical specimens.50 M.tuberculosis species were identified with PCR-LiPA technique.PCR-direct sequencing(PCR-DS) techniques were used as the control.Results:50 M.tuberculosis isolates performed traditional drug-susceptibility showed that there were 7 rifampicin resistant strains,12 Isoniazid resistant strains,15 streptomycin resistant strains and 2 ethambutol resistant strains.50 M.tuberculosis clinical isolates were detected by LiPA.4 rifampicin-resistant M.tuberculosis of 50 showed the mutation in rpoB genes,5 Isoniazid resistant M.tuberculosis of 50 showed the mutation in katG genes,7 streptomycin resistant M.tuberculosis of 50 showed the mutation in rpsL genes,5 ethambutol resistant M.tuberculosis of 50 showed the mutation in embB genes.The results from PCR-LiPA had 100% consistency with PCR-DS.Conclusion:PCR-LiPA might become a rapid,simple,sensitive and specific method to detect rpoB,katG,rpsL,rrs,embB genes mutations in part of drug-resistant M.tuberculosis.It could be used for clinical detection of drug-resistance as an assistant test.Read More
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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