Title: Studies on hydrolyzing protein of silver carp with neutral protease and acid protease
Abstract: In order to investigate basic properties of protein hydrolysis process of silver carp by neutral protease and acid protease,factors such as heat treatment,hydrolysis time,concentration of substrate,ammo acid content,hydrolyzing temperature,which have been generally reported to have important effect on enzymatic hydrolysis were studied.In addition,process of enzymaticaly hydrolyzing silver carp protein with the two proteases in two steps was investigated.As shown by the results,a preheat treatment for denature the fish protein allows for better hydrolysis;the optimum condition for enzymatic hydrolyzing silver carp protein with neutral protease is: hydrolysis temperature 40 ℃,pH7.0,protein concentration 6%,hydrolysis time 25 minutes,keeping the ammo acid concentration no more than 1.10 mg/mL.The optimum condition for enzymatic hydrolyzing silver carp protein with acid protease is: temperature 40 ℃,pH3.0,and protein concentration 6%,hydrolysis time 30 minutes and keeping the amino nitrogen concentration no more than 0.85 mg/mL.When the process of enzymatic hydrolysis protein of silver carp with two enzymes in two steps was adopted,the concentration of amino acid in hydrolysate was no more than that of hydrolyzing this fish protein with neutral protease.
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot