Title: Molecular cloning and expression of neurotrophin-3 gene and characterization of its expressed product
Abstract: With the chromosomal DNA of human blood lymphocytes as template,neurotrophin-3 coding gene was amplified by PCR and recombinated into phage vector M13mp18RF,which was sequenced by using single-stranded DNA terminal termination method. The sequence of the cloned gene is completely the same as that of the literature reported. The NT-3 coding gene was subcloned in a baculovirus expression vector. A large quantity of mature NT3 was detected in the culture medium after the insect cells in suspension culture were transfected by high molecular weight of DNA transposed in E. coli. on human neuroblastoma cell line SH-SY5Y-T3. The NT3 in expressed supernatant have marked biological effect which can be blocked by anti-human NT3 polyclonal antibody.
Publication Year: 2001
Publication Date: 2001-01-01
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot