Title: Selection of peptides that bind lipid A from a phage-displayed peptide library
Abstract: To select peptides that bind lipid A from a phage-displayed peptide library.Peptides were screened from a phage-displayed peptide dodecamer library by using lipid A as a ligand.After three rounds of biopanning,the e-luted phages were characterized by phagemid ELISA and DNA sequencing.14eluted clones were examined and could bind lipid A specifically,and amino acid sequences of random peptide dodecamers of4positive clones were identical.The results show that a peptide binding lipid A from phage-displayed peptide library has been selected.
Publication Year: 2003
Publication Date: 2003-01-01
Language: en
Type: article
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