Title: Research of Frequency Regenerated System Using Different Explants in Japonica Rice of Ningxia
Abstract: The effects of different explants culture were studied on influence of genotype and culture medium with four japonica rice cultivars.The research showed that different explants culture were different largely.Generally,young embryo was better than mature embryo,and anther was worst.Genotype impacted rice tissue culture greatly,Ningjing 34 was best effect of culture using mature embryos and young embryos,callus induction rates were 71.9% and 81.5% respectively,the green plantlet differentiation rates were 51.0% and 63.3% respectively;Ningjing 35 was best effect of culture using anther.The different culture medium and different genotypes were different culture effect.using mature embryos,Ningjing 16 and Ningjing 35 were better in NB culture medium;using young embryo,ningjing 35 and ningjing 38 were better in NB culture medium.Ningjing 35 was better culture effect in A4 culture medium using author,and compared with N6 culture medium,the green plantlet differentiation rate in A4 culture medium was remarkably improved.
Publication Year: 2008
Publication Date: 2008-01-01
Language: en
Type: article
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