Title: Inhibitory effects of Bauhinia championii flavones on cadiocytes apoptosis induced by H_2O_2
Abstract: AIM To observe the effects of flavones from Bauhinia championii(BCF) on H_2O_2-induced cardiomyocytes apoptosis in neonatal rats and its mechanisms.METHODS Peroxidative damage model was induced by adding 100 μmol·L~(-1)H_2O_2 in neonatal rat cardiomyocytes for 16 h.The rat cardiomycytes was randomly divided into normal group,model group,vitamin C(l00mg·L~(-1)) and BCF(50,100,200 mg·L~(-1))preconditioning groups.BCF and vitamin C were administrated for 2 h prior to H_2O_2 treatment.Cell viabilities were measured by MTT assay.Annexin V- FITC/PI staining was used to detect cell apoptosis.Tumor necrosis factor alpha(TNF-α) and total antioxidant activity(T-AOC) was determined by ELISA,and the protein expression of Bcl- 2 and Bax was observed by immunohistochemistry.RESULTS Compared with the model group,BCF pretreatment increased cell viability,T-AOC and the expression of Bcl-2(P 0.01 or P 0.05),while decreased cardiomyocytes apoptosis and the expression of TNF- a and Bax.There was no significant difference(P 0.05) between the vitamin C group and the BCF groups.CONCLUSION BCF inhibits H_2O_2-induced cardiomyocytes apoptosis,which might be mediated by decreasing lipid peroxidation,reducing TNF-a,up-regulating the expression of Bcl-2 and down-regulating Bax.
Publication Year: 2014
Publication Date: 2014-01-01
Language: en
Type: article
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