Abstract: Objective To investigate the effect of MDA/IL-24 on the human hepatocullular car- cinoma line Hep3B in vitro.Methods The MDA-7/IL-24 gene was transfected into human hepatocullular carcinoma cell line Hep3B and normal liver cell line L02 with an replication-incompetent adenovirus vec- tor.The expression of MDA7/IL-24 and bcl-2 in Hep3B and L02 ceils was detected by RT-PCR and ELISA assay.MTT assay and flow cytometry were used to study tumor cell proliferation and cell cycle.Ho- echst staining and Annexin-V and PI staining were performed to study MDA-7/IL-24 gene expressed in Hep3B and L02 cells.Results The protein concentration of MDA-7 in supematant of Hep3B and L02 cells was 790 and 810 pg/mL respectively.MDA-7/IL-24 could significantly inhibit the human hepatocel- lular carcinoma cell Hep3B growth (inhibition rate was 83% and 1.2%),promote the apoptosis (58% and 2.2%) and block the cells in G_2/M in vitro (48.29%,7.95%),but couldn't promote the apoptosis and inhibit the proliferation of L02 cells.The gene expression of bcl-2 was significantly deceased in Hep3B but not in L02 cells.Conclusion MDA-7/IL-24 selectively induces growth suppression and apoptosis of hepatocellular carcinoma lines Hep3B in vitro but not normal liver cell L02 by inhibiting the expression of bcl-2.
Publication Year: 2007
Publication Date: 2007-01-01
Language: en
Type: article
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