Title: Establishment of Balb/c nude mice transplantation tumor model with human breast cancer for study of gene therapy.
Abstract: Objective To establish a nude mice transplantation tumor model with human breast cancer for study of gene therapy.Methods MCF-7 Cells were grown in monolayer cell culture.30 nude mice were inoculated with 2×10~7 cells of MCF-7.These nude mice were divided into three groups: Lipofectin,NODN/Lip and ASODN/Lip.The observation was made by tumor restriction rate and apoptosis rate.Results The subcutaneous tumor model in nude mouse was successfully built.The overall rate of tumor implantation and growth was 100 percent in ASODN/Lip group;the restriction rate reached 70.10%;the apoptosis rate was 38.6%;and these rates were statistically different from the other two groups(P0.05).Conclusions This model is highly reproducible and is useful in the further study of breast cancer gene therapy.
Publication Year: 2005
Publication Date: 2005-01-01
Language: en
Type: article
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