Title: Cloning and Expression of Human APRIL_(105-250)Gene in E.coli
Abstract: Objective:To obtain human soluble APRIL cDNA and stable expression on E.coli DH5α.Methods: Screening the human APRIL protein sequence from GenBank(No:07588),designing the primer according to part of its extracelluar protein sequence.The total RNA,extracted from human tonsil,was amplified into the sequence of cDNA encoding sAPRIL by RT PCR and cloned into vector pUC18 for sequencing.Next,the identified cDNA was cloned into the new type of prokaryotic expression vector pQE 80L.Transmited the combined plasmids into E.coli DH5α and purifieded the protein by Ni 2+ .Results: The sAPRIL molecule was successfully expressed on E.coli DH5α and the protein was purifieded.Conclusion:The sAPRIL was expressed stably on E.coli DH5α.These works may provide evidence for the further study of the sAPRIL protein.
Publication Year: 2004
Publication Date: 2004-01-01
Language: en
Type: article
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