Title: STUDY THE EFFECT OF TANSHINONE IIA ON APOPTOSIS OF MYOCARDIAL CELLS OF ISCHEMIA-REPERFUSION RATS
Abstract: [Objective]To study the effect of tanshinone ⅡA on apoptosis of myocardial cells of ischemia-reperfusion rats.[Methods]50 Wistar rats were divided into 5 groups:sham operation group,ischemia-reperfusion group and high,moderate and low doses of tanshinone ⅡA group.By ligating the anterior descending branch of left coronary artery,model of myocardial ischemia and reperfusion was prepared.10min before ischemia,rats in 5 groups were respectively injected with 4ml saline,4ml saline,tanshinone ⅡA(16mg/kg,8mg/kg,4mg/kg)in 4ml saline.Observed the change the ECG after 45 min ischemia and 2h reperfusion;by immunohistochemistry to know the Bcl-2 and Bax protein expression in myocardial tissue.[Results]① The amplitude of T wave in ischemia reperfusion group was significantly higher compared with the sham group(P﹤0.01);T wave changes in different doses groups were decreased,compared with ischemia reperfusion group(P﹤0.05).② The incidence of 2h arrhythmia in ischemia-reperfusion group increased,compared with the sham group(P﹤0.01);The incidence of 2h arrhythmia in different doses groups decreased,compared with ischemia-reperfusion group(P﹤0.05).③ Bcl-2OD value,Bcl-2/Bax in ischemia reperfusion group were lower,compared with the sham group(P﹤0.01);Bcl-2OD value,Bcl-2/Bax in different doses groups were lower,compared with ischemia reperfusion group(P﹤0.05).BsxOD value in ischemia-reperfusion group was significantly higher compared with the sham group(P﹤0.01);BsxOD value in different doses groups was significantly lower than the ischemia reperfusion group(P﹤0.05).[Conclusion]Tanshinone ⅡA has significantly protective effect on myocardial cells of ischemia-reperfusion rates,the protective mechanism may up regulate Bcl-2 and down regulate Bax protein expression to inhibit the apoptosis of myocardial cells in ischemia-reperfusion.
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
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Cited By Count: 1
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