Title: Role of Nrf2-ARE signaling pathway in protective effect of hypoxia or pinacidil postconditioning against hypoxia-reoxygenation injury in adult rat cardiomyocytes
Abstract: AIM: To investigate whether nuclear factor E2-related factor 2(Nrf2)-antioxidant response element(ARE) signaling pathway is involved in the protective effect of hypoxia or pinacidil postconditioning against hypoxiareoxygenation injury in adult rat cardiomyocytes.METHODS: Cardiomyocytes were isolated from the left ventricle of male adult Sprague-Dawley rats(250 ~ 300 g) by Langendorff perfusion and collagenase II digestion.The cells were randomly divided into six groups(n = 8 in each group).The cells in N group were cultured for 22 h without any treatment.The cells in the other five groups were cultured for 20 h and then underwent 45 min of hypoxia followed by 60 min of reoxygenation(M group),three 5-min cycles of reoxygenation/hypoxia plus 60 min of reoxygenation(IPO group),or treatment with pinacidil(25,50 and 100 μmol / L) for 5 min plus 60 min of reoxygenation(P 25,P 50 and P 100 groups,respectively).The expression of Nrf2,NAD(P) H: quinone oxidoreductase 1(NQO1),heme oxygenase 1(HO-1) and superoxide dismutase 1(SOD1) at mRNA and protein levels was detected by real-time PCR and Western blotting,respectively.RESULTS: The mRNA and protein levels of Nrf2,HO-1,NQO1 and SOD1 in M,IPO,P 25,P 50 and P 100 groups were significantly decreased compared with N group(P 0.05),and those in IPO,P 25,P 50 and P 100 groups were obviously higher than those in M group(P 0.05).The mRNA levels of Nrf2,HO-1,NQO1 and SOD1 in IPO and P 50 groups were obviously higher than those in P 25 and P 100 groups(P 0.05).The mRNA expression of SOD1 in P 25 group was obviously higher than that in P 100 group(P 0.05).The protein levels of Nrf2,NQO1 and SOD1 in IPO and P 50 groups were obviously higher than those in P 25 and P 100 groups(P 0.05).The protein expression of HO-1 in P 50 group was obviously higher than that in IPO,P 25 and P 100 groups(P 0.05).The protein levels of HO-1 and NQO1 in P 25 group were obviously higher than those in P 100 group(P 0.05).CONCLUSION: Hypoxia or pinacidil postconditioning may attenuate hypoxia-reoxygenation injury in adult rat cardiomyocytes via activation of Nrf2-ARE signaling pathway.
Publication Year: 2013
Publication Date: 2013-01-01
Language: en
Type: article
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Cited By Count: 1
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