Title: Effects of tetramethylpyrazine on expression of Bcl-2 and Fas proteins in myocardial injury induced by catecholamine in rats
Abstract: AIM: To observe the influence of tetramethylpyrazine on the ex pression of Bcl-2 and Fas proteins in myocardial injury induced by catecholamin e in rats. METHODS: 36 Wistar rats were divided into 3 groups: c ardiac injury group (n=12), tetramethylpyrazine treated group ( n=12) and control group (n=12).The rat card iac injury was induced by the subcutaneous injection of isoproterenol (ISO, 5 mg·kg -1 ·d -1 ). Bcl-2 and Fas protein was identified by immunoh istochemical technique and the mean optical density (OD) values of the positive fields of protein expression were quantitatively examined by ima ge analysis system. RESULTS: The expression of Bcl-2 protein wa s increased significantly in the cardiac injury group and tetramethylpyrazine tr eated group as compared with that of the control group (P 0.0 1 ). There were no significant difference in the Bcl-2 protein expression betw een the tetramethylpyrazine treated group and cardiac injury group (P 0.05 ), but the expression of Fas protein was decreased significantly i n the tetramthylpyrazine treated group as compared with that of the cardiac inju ry group (P 0.05 ). The protein expression ratio of Bcl-2/Fa s was increased significantly in the tetramethylpyrazine treated group as compar ed with those of the cardiac injury group and the control group.The myocardium pathological damage was decreased significantly in the tetramethylpyrazine treat ed group. CONCLUSION: Tetramethylpyrazine can significantly inhi bit the expression of pro-apoptotic Fas protein and raise the ratio of Bcl-2/F as protein cardiomyocytes.
Publication Year: 2004
Publication Date: 2004-01-01
Language: en
Type: article
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Cited By Count: 2
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