Title: Determination of Icariin in JieXinTong Capsules by RP-HPLC
Abstract: [Objective] To establish a method for determining the content of icariin in JieXinTong capsules by RP-HPLC.[Methods]An RP-HPLC method was set up to determine the icariin by using Hypersil ODS C18(4.6 mm×250 mm,5μm)column,and acetonitrile-0.2%phosphoric acid(30︰70)as mobile phase.The UV detection wavelength was 270 nm and the flow rate being 1.0 ml/min.[Results] The calibration curve was showed good linearity(r=0.9998,n=5)within the range from 0.045 to 0.360μg and the average recovery was 99.6%,RSD was 0.77%(n=6).[Conclusions]The method is simple,and with good reproducibility for the determination of icariin in JieXinTong capsules.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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