Title: Optimization of bacteriocin production by Lactobacillus plantarum 8-6
Abstract: Fermentation conditions of bacteriocin produced by Lactobacillus plantarum 8-6 were optimized.Effects of culture time,temperature,inoculum,initial pH value,carbon source and nitrogen on bacteriocin production were studied.The optimum media composition and fermentation conditions were obtained by single-factor and orthogonal experiments as follows: glucose 3%,tryptone 1.5%,peptone 1.5%,yeast extract 1%,magnesium sulfate 0.058%,tween-80 0.2%,culture temperature 30℃,culture time 28h,initial pH value 6.5 and inoculum 2%.Under the above conditions,the production of bacteriocin was 1825.56 IU/ml,which increased by 373.15% compared to that before optimization.
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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