Title: Construction of Lentiviral Expression Vector of CXCR4 and Its Expression in Human Umbilical Cord Mesenchymal Stem Cell
Abstract: Objective: To construct the lentiviral expression vector of CXCR4 and study the expression in human umbilical cord mesenchymal stem cell(hUCMSCs). Methods: A reverse transcription-polymerase chain reaction( RT-PCR) was performed to get CXCR4 gene code fragment, 293T cells were co-transfected with lentiviral expression vector of CXCR4, psPAX2 packaging plasmid and pMD2.G. The plasmid was coated to pack lentivirus. Then lentiviral expression vector of CXCR4 were co-transfected with hUCMSCs and the expression of CXCR4 mRNA and protein expression level were detected by RT-PCR and western blot respectively. Results: By means of sequencing, restriction endonuelease analysis and PCR, lentiviral expression vector of CXCR4 has been successfully constructed.lentiviral expression vector of CXCR4 co-transfected with hUCMSCs can effectively increase the expression of CXCR4 in MSCs.Conclusions: Lentiviral expression vector of CXCR4 co-transfected with hUCMSCs has been successfully constructed, which can be applied to further research of MSCs.
Publication Year: 2013
Publication Date: 2013-01-01
Language: en
Type: article
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