Title: Study of Y chromosome azoospermia factor microdeletions in infertile patients with Klinefelter's syndrome.
Abstract:Objective: To observe spermatogenesis and the microdeletions of AZF(azoospermia factor) on Y chromosome in infertile male with Klinefelter's syndrome(KS).Methods: Testicular cytology by fine neddle as...Objective: To observe spermatogenesis and the microdeletions of AZF(azoospermia factor) on Y chromosome in infertile male with Klinefelter's syndrome(KS).Methods: Testicular cytology by fine neddle aspiration(FNA) and Y chromosome microdeletions were performed in 12 KS patients to carried out pre-ICSI/IVF.Multiplex PCR was used to detect the AZF-candidate genes as follows:sY84,sY86,sY127,sY134,sY152,sY153,sY254,sY255,and ZFX/Y was used as internal control gene.Thirty-two healthy fertilized men used as controls.Results: Cytology analysis by FNA in 12 patients with KS revealed a little of spermatozoa with normal morphology and different levels spermatogenic cell and the spermatids in 3 KS,only spermatogenic cells in 7 KS and spermatogenesis arrest in 2 KS.Two microdeletion cases existed in total 12 KS,including one in AZFa+ AZFc and one in AZFc+ AZFb.No microdeletion was detected in fertile controls(χ2=5.587,P=0.018).Conclusion:AZF region microdeletion of Y chromosome might account for about 16.7% of infertile male patients with KS.Read More
Publication Year: 2008
Publication Date: 2008-01-01
Language: en
Type: article
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