Title: Anti-tumor Activity of Extract from Periploca Sepium Bge
Abstract: BACKGROUND AIM: To investigate the anti-tumor activity of extract from Periploca Sepium Bge(PSBE)and to study its probable mechanisms. MATERIALS AND METHODS: The growth-inhibitory effect of ethanol extract from Periploca sepium bge(PSBEE)and water extract from Periploca sepium Bge(PSBWE)on MCF-7,TE-13,QG56,SMMC7721,T24,Hela,K562tumor cell lines were examined by using MTT assay,the change of cell cycle and the induction of apoptosis were analyzed with flow cytometry. RESULTS: Both PSBEE and PSBWE could significantly inhibit the proliferation of tumor cell lines,the effect was dose-dependent,PSBEE(IC 50 10μg/ml)was more effective than PSBWE(IC 50 40μg/ml).PSBEE could arrest the cell cycle at G 0/G 1 phase and induce apoptosis in MCF-7cells in a time-dependent manner.The proportion of tumor cells in the G 0/G 1 phase increased after treatment with10μg/ml PSBEE for24hours,the apoptotic rate reached13.54%±2.12%after48h. CONCLUSION: Cell growth in vitro was inhibited significanttly by extract from Periploca sepium Bge,PSBEE might exert its anti-tumor activity by blocking the cell cycle and inducing apoptosis of tumor cells.
Publication Year: 2006
Publication Date: 2006-01-01
Language: en
Type: article
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Cited By Count: 4
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