Title: Phagocytosis of IgA immune complexes by human U937 cells
Abstract: Objective Studying FcαRⅠ mediated phagocytosis of IgA immune complexes by U937 cells. Methods Antigen 8.9NIP/BSA was labeled with FITC and then reacted with anti NIP IgA or anti NIP IgG antibodies to form immune complexes (IC). They were then incubated with phorbol 12-myristate 13-acetate (PMA) stimulated U937 cells. The phagocytosed IC was quantified by flow cytometry. Results Expression of FcαRⅠ on U937 cells was higher than that of FcγRⅠ, FcγRⅡ and FcγRⅢ. After stimulation by PMA, expression of FcαRⅠ on U937 cells was markedly upregulated and the phagocytosis of IgA IC was enhanced. FcαRⅠ mediated specific IgA phagocytosis was stronger than FcγRⅠ and FcγRⅡ mediated IgG phagocytosis. Complement receptors, CR1 and CR3, enhanced U937 cell phagocytosis of IgA IC. Conclusion FcαRⅠ mediated strong phagocytosis of IgA IC.
Publication Year: 2003
Publication Date: 2003-01-01
Language: en
Type: article
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