Title: Effects of atorvastatin on expressions of ERK,p38MAPK,NF-κB proteins and ICAM-1 mRNA in HUVECs induced by endothelial microparticles
Abstract: Aim:To observe the effects of atorvastatin on ERK,p38MAPK and nuclear factor-κB(NF-κB) and intercellular adhesion molecule 1(ICAM-1) expressions in endothelial microparticles(EMPs)-induced human umbilical vein endothelial cells(HUVECs).Methods:The cultured HUVECs were cultured with EMPs of 105 mL-1for 0,3,6,12,and 24 h.The HUVECs were cultured with 0,102,103,104,105 mL-1 EMPs for 24 h.The HUVECs were pretreated with ERK inhibitor(PD98059),p38 inhibitor(SB203580),NF-κB inhibitor(PDTC),or atorvastatin,then were treated with EMPs.The ICAM-1 mRNA level was detected by Real-time-PCR.Western blot was performed to determine the expression levels of phospho-ERK(p-ERK),p-p38MAPK and NF-κB p65 proteins.Results:With the increase of dose and exposure time of EMPs,the expression levels of ICAM-1 mRNA,p-ERK,p-p38MAPK and NF-κB p65 protein in HUVECs obviously increased(P0.001).After the pretreatment of the inhibitors mentioned above and atorvastatin,the ICAM-1 mRNA expression decreased(P0.05).Conclusion:Atorvastatin could downregulate ICAM-1 expression in EMPs-induced HUVECs by blocking ERK/MAPK and NF-κB signaling pathway.
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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