Title: Effect of resveratrol on cell cycle and apoptosis in 3T3-L1 preadipocytes
Abstract:Objective To study the effects and mechanism of resveratrol(Res) on apoptosis and cell cycle in 3T3-L1 preadipocytes.Methods 3T3-L1 preadipocytes were treated with different doses of Res treatment(0,2...Objective To study the effects and mechanism of resveratrol(Res) on apoptosis and cell cycle in 3T3-L1 preadipocytes.Methods 3T3-L1 preadipocytes were treated with different doses of Res treatment(0,25,50,75,and 100 μmol / L,0 μmol / L as the negative control) for 24,48 or 72 h.The fluorescence microscope,MTT assay,and flow-cytometry were used for measuring cell morphology,cell proliferation,cell cycle,and apoptosis.Results After treated with 50 μmol / L of Res for 48 h,3T3-L1 preadipocytes exhibited typical apoptosis features when observed under fluorescence microscope.MTT assay showed that,after different doses of Res treatment(0,25,50,75,and 100 μmol / L) for 24 h,cell proliferation rate was 100%,(97.00 ± 1.00) %,(91.00 ± 2.65) %,(90.67 ± 2.52) %,and(86.00 ±3.61) %,respectively;after the similar Res treatment for 48 h,cell proliferation rate was 100%,(86.67 ±2.52) %,(76.00 ±2.00) %,(34.33 ±2.08) %,and(30.33 ±2.52) %,respectively;also,after the similar Res treatment for 72 h,cell proliferation rate was 100%,(82.00 ± 2.65) %,(65.67 ±3.06) %,(21.00 ±3.61) %,and(16.33 ±3.21) %,respectively.Partial correlation analysis showed a negative correlation between cell proliferation rate and intervention time,concentration of Res(r =-0.72、-0.83,all P 0.01).After different doses of Res treatment(0,25,50,75,and 100 μmol / L) for 24 h,the portion of cells in G0 / G1 phase was(27.23 ± 2.63) %,(39.03 ± 2.74) %,(80.20 ±5.15) %,(87.97 ±3.12) %,(90.80 ±2.08) %,respectively;as the same,the portion of cells in S phase was(72.43 ± 2.99) %,(63.93 ± 6.90) %,(19.80 ± 5.15) %,(12.20 ± 2.86) %,(9.20 ±2.08) %,respectively.From the results of the cell cycle examination,the portion of cells in G0 / G1 phase decreased(P 0.05 or P 0.01) and increased evidently in S phase(P 0.05 or P 0.01) in a dose dependent manner.After different doses of Res treatment(0,25,50,75,100 μmol / L) for 48 h,apoptosis rate was(2.90 ± 0.10) %,(5.40 ± 3.81) %,(8.23 ± 4.24) %,(29.77 ± 6.18) %,(27.23 ±3.17) %,respectively;as the same,necrosis rate was(7.50 ± 0.87) %,(12.00 ± 4.89) %,(12.27 ±3.81) %,(12.67 ±6.13) %,(20.73 ± 2.64) %,respectively.The apoptosis and necrosis of 3T3-L1 preadipocytes were statistically significant different between 100 μmol / L Res group and negative control group(P 0.05 or P 0.01).Conclusion At a certain dose range(0-100 μmol / L),Res may inhibit the life cycle and promote apoptosis of 3T3-L1 preadipocytes.Read More
Publication Year: 2013
Publication Date: 2013-01-01
Language: en
Type: article
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