Title: Sodium cantharidate induced gene expression changes in human erytroleukemia K562 cells
Abstract: Objective:To investigate the apoptosis of human erythroleukemia K562 cells and identify the difference in gene expression profile of K562 cells induced by sodium cantharidate.Methods: Saline as negative control,sodium cantharidate of different concentration was added to K562 cells,then MTT assay and DNA gel electrophoresis were carried out to detect the cell apoptosis.Total RNA of the pre-and post-treatment K562 cells was extracted and tuned into cDNA by reverse transcription.Experimental and control samples were labeled by Cy5-dCTP and Cy3-dCTP respectively in this course.Then these samples were hybridized to microarrays which contain 162 cDNA oligos.Results: Significant growth inhibition occurred in K562 cells in vitro after sodium cantharidate treated,and 2.5μg/ml sodium cantharidate could induce apoptosis.It's also found that among the 540 spotted microarray,37 genes differently expressed,including transcription factors,tRNA synthetase,metabolic emzymes,signal transduction proteins and kinases.Conclusion: Sodium cantharidate could induce K562 cells apoptosis efficiently,and the cDNA microarray constructed by us could successfully be applied to analyze the expression profile of genes.
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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